MHCD3819

antibody from Invitrogen Antibodies

Targeting: CD38

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MHCD3819

Provider

Invitrogen Antibodies

Product name

CD38 Monoclonal Antibody (HIT2), APC-Cyanine5.5

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

HIT2

Vial size

500 µL

Storage

4° C, store in dark

References

Submitted references

Distinct immunological signatures discriminate severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia.

Kreutmair S, Unger S, Núñez NG, Ingelfinger F, Alberti C, De Feo D, Krishnarajah S, Kauffmann M, Friebel E, Babaei S, Gaborit B, Lutz M, Jurado NP, Malek NP, Goepel S, Rosenberger P, Häberle HA, Ayoub I, Al-Hajj S, Nilsson J, Claassen M, Liblau R, Martin-Blondel G, Bitzer M, Roquilly A, Becher B
Immunity 2021 Jul 13;54(7):1578-1593.e5

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Polyreactive Broadly Neutralizing B cells Are Selected to Provide Defense against Pandemic Threat Influenza Viruses.

Guthmiller JJ, Lan LY, Fernández-Quintero ML, Han J, Utset HA, Bitar DJ, Hamel NJ, Stovicek O, Li L, Tepora M, Henry C, Neu KE, Dugan HL, Borowska MT, Chen YQ, Liu STH, Stamper CT, Zheng NY, Huang M, Palm AE, García-Sastre A, Nachbagauer R, Palese P, Coughlan L, Krammer F, Ward AB, Liedl KR, Wilson PC
Immunity 2020 Dec 15;53(6):1230-1244.e5

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Human C. difficile toxin-specific memory B cell repertoires encode poorly neutralizing antibodies.

Shah HB, Smith K, Scott EJ 2nd, Larabee JL, James JA, Ballard JD, Lang ML
JCI insight 2020 Aug 20;5(16)

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Identification of a human monoclonal antibody to replace equine diphtheria antitoxin for treatment of diphtheria intoxication.

Sevigny LM, Booth BJ, Rowley KJ, Leav BA, Cheslock PS, Garrity KA, Sloan SE, Thomas W Jr, Babcock GJ, Wang Y
Infection and immunity 2013 Nov;81(11):3992-4000

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Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen.

Smith K, Garman L, Wrammert J, Zheng NY, Capra JD, Ahmed R, Wilson PC
Nature protocols 2009;4(3):372-84

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Polyreactivity Is Inherent to Naive B Cells Selected into the Broadly Neutralizing Response (A) Proportion of mAbs generated from total naive B cells and MBCs and from influenza stalk domain-binding germline B cells, MBCs, and plasmablasts that were polyreactive. (B and C) Binding AUC of polyreactive mAbs or the corresponding germline-reverted mAbs binding to A/California/7/2009 (n = 12) and A/Brisbane/59/2007 viruses (n = 13) (B) and dsDNA (n = 11), insulin (n = 12), and LPS (n = 12) (C). Each line connects the germline and affinity-matured version of the same mAb. (D and E) Fold change in AUC of affinity-matured mAbs over AUC of germline mAbs of all tested mAbs (D) and further broken down by initial germline affinity for A/California/7/2009 virus (E). Cyan dots represent sc70 1F02, and orange dots represent SFV005 2G02. (F and G) MD simulations of the HC-CDR3 sequences of germline and affinity-matured versions of mAbs sc70 1F02 (F) and SFV005 2G02 (G). For (A), the numbers on top of individual bars is the number of polyreactive mAbs out of total mAbs tested from each cohort. For (D) and (E), each symbol represents one mAb, and the red bar represents the median. Data in (A) were analyzed by Fisher's exact test, and data in (B) and (C) were analyzed by paired non-parametric Wilcoxon matched-pairs signed rank tests. See also Figure S6 .