- 13-0389-82 - Invitrogen Antibodies CD38 antibody

Submitted references Immunometabolic interference between cancer and COVID-19.

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Frontiers in immunology 2023;14:1168455

Novel myeloma patient-derived xenograft models unveil the potency of anlotinib to overcome bortezomib resistance.

Yue Y, Cao Y, Mao X, Wang F, Fan P, Qian L, Guo S, Li F, Guo Y, Chen T, Lin Y, Dong W, Liu Y, Huang Y, Gu W
Frontiers in oncology 2022;12:894279

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Enhanced CXCR4 Expression of Human CD8(Low) T Lymphocytes Is Driven by S1P(4).

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Frontiers in immunology 2021;12:668884

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PLoS pathogens 2021 Jan;17(1):e1009179

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

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PLoS pathogens 2021 Apr;17(4):e1009117

Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases.

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EBioMedicine 2020 Aug;58:102904

IFNγ induces epigenetic programming of human T-bet(hi) B cells and promotes TLR7/8 and IL-21 induced differentiation.

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Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

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Su KY, Watanabe A, Yeh CH, Kelsoe G, Kuraoka M
Journal of immunology (Baltimore, Md. : 1950) 2016 Nov 15;197(10):4163-4176

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Fernandez I, Ooi TP, Roy K
Stem cells (Dayton, Ohio) 2014 Jan;32(1):93-104

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

Radomska HS, Alberich-Jordà M, Will B, Gonzalez D, Delwel R, Tenen DG
The Journal of clinical investigation 2012 Aug;122(8):2955-66

CD38 binding to human myeloid cells is mediated by mouse and human CD31.

Horenstein AL, Stockinger H, Imhof BA, Malavasi F
The Biochemical journal 1998 Mar 15;330 ( Pt 3):1129-35

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or Anti-Human CD38 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4. IFNgamma is required for development of T-bet hi B DN cells and regulates ASC formation and recovery. ( a ) Ingenuity Pathway Analysis (IPA) to identify predicted upstream direct and indirect regulators of the HD B DN Be1 transcriptome. IPA performed using the 427 DEG (B DN Be1 over B DN Be2; FDR < 0.05) identified in the RNA-seq analysis described in Figure 3b . The predicted activation state (z-score of B DN Be1 over B DN Be2) of each regulator/signaling pathway is shown as bar color (orange, activated; blue, inhibited) with predicted upstream regulators sorted in order of significance (overlap P value). Regulators with an overlap P -value
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5. Temporally distinct regulation of T-bet hi IRF4 int pre-ASC and ASC development by IFNgamma, R848 and IL-21. Cartoon ( a ) depicting stimulation of CTV-labeled HD B N cells for 3 days with anti-Ig, R848, IL-21 and IFNgamma (Step 1). Cells were washed and re-cultured for 3 days with R848, IFNgamma, and IL-21 (Step 2, +,+ condition) or individual stimuli were included in Step 1 only (+,- condition) or in Step 2 only (-,+ condition). Cells from day 6 cultures containing IFNgamma ( b-e ), R848 ( f-i ) or IL-21 ( j-m ) in Step 1, Step 2 or both steps were analyzed to determine ASC frequencies ( b, f, j ), ASC recovery ( c, g, k ), cell division ( d, h, l ) and total cell recovery ( e, i, m ). Summary of data ( n ) showing that ASC development and recovery from T-bet hi IRF4 int B DN pre-ASCs requires early IFNgamma, R848 and BCR ''priming'' signals and late R848 and IL-21 proliferation and differentiation signals. See Figure 5-figure supplement 1 for representative flow cytometry plots from each culture showing T-bet hi IRF4 int B DN cells on day 3, CD38 hi CD27 + ASCs on day 6 and CTV dilution on day 6. Data are representative of >=3 experiments. The percentage of cells in each division, the frequency of ASCs and cell recovery (total and ASCs) are shown as the mean +-SD of cultures containing purified B N cells from 3 independent healthy donors. All statistical analyses were performed using one-way ANOVA with Tukey''s multiple comparison test. P values *
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6-figure supplement 2. Flow cytometric characterization of B cells activated during the early priming phase in the presence or absence of IFNgamma and IL-2. Day 6 Be.0, Be.IL2, Be.IFNgamma and Be.gamma2 cells were generated as described in Figure 6e . Representative flow cytometry plots from day 6 Be.0, Be.IFNgamma, Be.IL2 and Be.gamma2 cultures showing CD38 hi CD27 + ASCs ( a ) and CTV dilution ( b ).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6. IFNgamma cooperates with R848, IL-2 and IL-21 to promote development and recovery of ASCs. ( a-d ) IFNgamma synergizes with subthreshold amounts of TLR7/8 ligand to induce proliferation and differentiation of B N cells. CTV-labeled HD B N cells were activated for 3 days (Step 1) with anti-Ig, IL-2, and increasing concentrations of R848 (as indicated) in the presence or absence of IFNgamma (10 ng/ml). Cells were washed and re-cultured for 3 additional days (Step 2) with IL-21 and the same concentration of R848 that was used in Step 1. B cell division was measured on day 6 in cultures that were activated with IFNgamma (green circles) or without IFNgamma (orange circles) in the presence of no R848 (0 mug/ml, ( a ), high dose R848 (10 mug/ml, ( b ) or low dose R848 (0.1 mug/ml, ( c ). The frequency of CD38 hi CD27 + ASCs ( d ) on day 6 is shown. ( e-i ) IFNgamma cooperates with IL-2 to promote ASC development and recovery. Cartoon ( e ) depicting CTV-labeled HD B N cells activated for 3 days (Step 1) with anti-Ig and R848 alone (Be.0); with anti-Ig +R848+IFNgamma (Be.IFNgamma); with anti-Ig +R848+IL-2 (Be.IL2); or with anti-Ig +R848+IFNgamma+IL-2 (Be.gamma2). Cells were then washed and recultured for an additional 3 days (Step 2) with R848 and IL-21. The percentage of cells that have undergone cell division ( f ), the total cell recovery ( g ), the ASC frequencies ( h ) and total ASCs recovered ( i ) from each day 6 culture are shown. ( j-k ) Early IFNgamma signals regu
Conjugate: Biotin

Supportive validation

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Experimental details: Fig 4 p16 INK4a is a functional barrier to EBV driven proliferation of lymphoblastoid cells. (A) Blueprint of the primary transcript and the spliced mRNA with the three exons of CDKN2A on chromosome 9 encoding the p16 INK4a protein. The target site of the RNP complex within the 1st exon (exon1alpha) (chr9:21,974,678-21,974,827) is shown. (B) Study of the biological effect of the CDKN2A knockout in a time course experiment. WT and p16 KO cells were mixed such that the fraction of the latter was in the order of 10 to 20%, when the cells were infected with WT or DeltaEBNA3C EBV strains. The knockout status of the CDKN2A gene was studied by next generation sequencing to analyze the CD46 locus of the mixed cell populations over time. The fraction of cells with a disabled CDKN2A gene increased in cells infected with DeltaEBNA3C EBV exceeding 80% after eight weeks, whereas the knockout status of CDKN2A in the population of cells infected with WT EBV did not show a clear trend. Results from two biological replicates are shown, additional replicates can be found in S4A Fig . (C) Cell numbers of four different B cell populations were plotted as a function of days post nucleofection (x-axis) versus the format of the cell culture vessel (y-axis) starting with a single well in a 48-well cluster plate. 2x10 6 B cells with an intact CDKN2A locus (WT cells) or cells with an edited CDKN2A gene (p16 KO cells) were infected with wild-type (WT) EBV (left panel) or DeltaEBNA3C EBV (right panel).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 7 Increased exhausted CD8 + T cells in convalescent patients. A. Expression of activation marker CD38 on CD8 + T cells (upper FACS panel). One exemplary dot plot is shown per study group. The bar diagram (lower panel) shows that CD38 expression was significantly higher on CD8 + T cells in convalescent COVID-19 + patients compared with HC. *P-value
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: A schematic overview of our experimental design to generate antigen-specific CD8+ T cells. CD34+ CB cells were expanded for 7 days and CD34+CD38-/low cells were isolated. After 7 days of CD34+ cell expansion, CD34+ cells were enriched using magnetic bead separation. The enriched cells were stained with anti-CD34 and anti-CD38 antibodies to isolate the CD34+CD38-/low HSC population. 81.0% of FSC versus SSC gated cells were CD34+CD38-/low. Gate was determined by the isotype staining control. Data are representative of at least six independent experiments. To induce Notch signaling and early T cell differentiation, CD34+CD38-/low cells were seeded onto DLL1-coated nontissue culture-treated plates. HLA-TCR signaling to direct antigen-specific differentiation was induced using CMVpp65 peptide or epitope-loaded HLA-A*0201 class I tetramers or GIL epitope-loaded HLA-A*0201 class I tetramers. Abbreviations: CM, conditioned medium; CMV, cytomegalovirus; FSC, Forward Scatter; GIL, Influenza M1 virus; HLA, Human leukocyte antigen; SSC, Side Scatter; TCR, T cell receptor.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 7 FIGURE B cells from aged donor mice do not have defects in participating in the GC 10 days after immunization in young recipient mice and fewer B cells from young donor mice are recovered when transferred into aged recipient mice. (a) Representative flow cytometric plots of CD38 - CD95 + GC B cells gated on donor cells from 10-week-old or 93-week-old donor mice in recipient spleens on day 10 post-transfer and immunisation. Numbers adjacent to gates indicate percentage of donor HEL + B220 + cells. Percentage and number of donor-derived GC B cells (Donor HEL + B220 + CD38 - CD95 + ) are plotted on the graphs on the right. (b) Representative flow cytometric plots of IgM + and IgG1 + donor HEL + B cells. Numbers adjacent to gates indicate percentage of donor HEL + B220 + cells. (c, d) Percentage and number of (c) IgM + and (d) IgG1 + B cells derived from donor cells from young or aged mice in recipient spleens 10 days post-transfer and immunisation. (e) Graph showing the percentage and number of IgG1 + GC B cells out of total Donor HEL + B220 + CD38 - CD95 + cells. (f) Schematic diagram of adoptive transfer of B cells from young SW HEL mice into young (8-12 weeks old) or aged (>90 weeks old) mice in which B cells response was analysed 6 days post-transfer and immunisation. (g) Percentage and number of donor HEL + B220 + cells in spleens of young or aged recipient mice 6 days post-transfer and immunisation. (h) Percentage and number of donor-derived GC B cells (Donor HEL + B220
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Morphological and phenotypic features of tumor cells from newly established myeloma PDX. MM.1S cells and mononuclear cells isolated from BM and pleural effusion samples of MM patient were inoculated subcutaneously in NDG mice. When tumors reached 6-8 mm in diameter, tumors were removed and prepared for HE staining, IHC staining, and single-cell suspensions. (A) Representative image of myeloma MM.1S and PDX models. (B) The morphology of tumor cells from myeloma MM.1S and PDX models by Giemsa staining. Scale bar: 5 mum. (C) Representative IHC stainings of tumor tissues. Scale bar: 100 mum. (D) The single-cell suspensions were analyzed by flow cytometry. The doublet or aggregated events were gated out according to side scatter area (SSC-A) and side scatter width (SSC-W). 7-AAD staining was used to gate out dead cells. The expression levels of CD138, CD38, kappa, and lambda were analyzed in mouse CD45 - cells. Myeloma cell line MM.1S was used as a positive myeloma cell control. The left panels of flow charts were isotype controls. PDX, patient-derived xenograft; HE, hematoxylin-eosin; IHC, immunohistochemical.
Conjugate: Biotin

13-0389-82

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