Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 61-0389-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD38 Monoclonal Antibody (HIT2), PE-eFluor 610, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity. Applications Reported: This HIT2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HIT2
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.
Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.
CD38 binding to human myeloid cells is mediated by mouse and human CD31.
Douam F, Hrebikova G, Albrecht YE, Sellau J, Sharon Y, Ding Q, Ploss A
Nature communications 2017 Mar 14;8:14781
Nature communications 2017 Mar 14;8:14781
Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.
Kong F, Feng B, Zhang H, Rao H, Wang J, Cong X, Wei L
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Experimental and therapeutic medicine 2017 Aug;14(2):1846-1852
Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.
Fernandez I, Ooi TP, Roy K
Stem cells (Dayton, Ohio) 2014 Jan;32(1):93-104
Stem cells (Dayton, Ohio) 2014 Jan;32(1):93-104
Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.
Radomska HS, Alberich-Jordà M, Will B, Gonzalez D, Delwel R, Tenen DG
The Journal of clinical investigation 2012 Aug;122(8):2955-66
The Journal of clinical investigation 2012 Aug;122(8):2955-66
CD38 binding to human myeloid cells is mediated by mouse and human CD31.
Horenstein AL, Stockinger H, Imhof BA, Malavasi F
The Biochemical journal 1998 Mar 15;330 ( Pt 3):1129-35
The Biochemical journal 1998 Mar 15;330 ( Pt 3):1129-35
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (blue histogram) or Anti-Human CD38 PE-eFluor® 610 (purple histogram). Cells in the lymphocyte (left) and monocyte (right) gates were used for analysis.