MA5-15570

antibody from Invitrogen Antibodies

Targeting: CD38

ELISA

Antibody data

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Validation data

Product number: MA5-15570 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD38 Monoclonal Antibody (6E12D)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15570 targets CD38 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15570 immunogen is purified recombinant fragment of human CD38 expressed in E. Coli. MA5-15570 detects CD38 which has a predicted molecular weight of approximately 34kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 6E12D
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CD38 protein structure - MA5-15570 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of Molt-4 (Lane 1), Jurkat (Lane 2), Raji (Lane 3), Ramos (Lane 4), THP-1 (Lane 5) and U-937 (Lane 6). The blot was probed with Anti- CD38 Monoclonal Antibody (Product # MA5-15570, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). A 45 kDa band corresponding to CD38 was observed across all the cell lines tested except THP-1 and U-937.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD38/cyclic ADP-ribose hydrolase using CD38/cyclic ADP-ribose hydrolase monoclonal antibody (Product # MA5-15570) in CD38-human IgG Fc transfected HEK293 cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using CD38 Monoclonal Antibody (6E12D) (Product # MA5-15570) and a ~46 kDa band corresponding to CD38 was observed in Ramos, Daudi, A549, MOLT-4, Jurkat, Raji . Whole cell extracts (30 µg lysate) of Ramos (Lane 1), Daudi (Lane 2), A549 (Lane 3), MOLT-4 (Lane 4), Jurkat (Lane 5), Raji (Lane 6), THP-1 (Lane 7), H1299 (Lane 8), HCT 116 (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).