PA1-1742

antibody from Invitrogen Antibodies

Targeting: ADAMTS5 ADAMTS11, ADMP-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-1742

Provider

Invitrogen Antibodies

Product name

ADAMTS5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-1742 detects ADAMTS5 in human samples.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of ADAMTS5 in JJ012 cells using Product # PA1-1742.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADAMTS5 was performed by loading 25 µg of human brain (lane 1) and human heart (lane 2) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with an ADAMTS5 polyclonal antibody (Product # PA1-1742) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~64 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ADAMTS5 using a polyclonal antibody (Product # PA1-1742).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ADAMTS5 using a polyclonal antibody (Product # PA1-1742).