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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-32142 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAMTS5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, PC-12, Rat-2.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ADAMTS5 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ADAMTS5 Polyclonal Antibody (Product # PA5-32142) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ADAMTS5 Polyclonal Antibody (Product # PA5-32142). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2 . 7.5% SDS PAGE. ADAMTS5 Polyclonal Antibody (Product # PA5-32142) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using ADAMTS5 (Product # PA5-32142) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ADAMTS5 Polyclonal Antibody detects ADAMTS5 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatoma. ADAMTS5 Polyclonal Antibody (Product # PA5-32142) dilution: 1:250. Scale bar = 100 µm. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
