Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- H00009022-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009022-M02, RRID:AB_537837
- Product name
- Mouse Monoclonal CLIC3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CLIC3 - chloride intracellular channel 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLIC3 Antibody (3F8) [H00009022-M02] - Analysis of CLIC3 expression in transfected 293T cell line by CLIC3 monoclonal antibody (M02), clone 3F8.Lane 1: CLIC3 transfected lysate(26.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLIC3 Antibody (3F8) [H00009022-M02] - CLIC3 monoclonal antibody (M02), clone 3F8 Analysis of CLIC3 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CLIC3 Antibody (3F8) [H00009022-M02] - Detection limit for recombinant GST tagged CLIC3 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CLIC3 Antibody (3F8) [H00009022-M02] - Analysis of monoclonal antibody to CLIC3 on HeLa cell. Antibody concentration 15 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CLIC3 Antibody (3F8) [H00009022-M02] - Analysis of CLIC3 transfected lysate using anti-CLIC3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLIC3 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC3 Antibody (3F8) [H00009022-M02] - Analysis of monoclonal antibody to CLIC3 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC3 Antibody (3F8) [H00009022-M02] - Analysis of monoclonal antibody to CLIC3 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.