Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-89465 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89465, RRID:AB_11028869
- Product name
- Rabbit Polyclonal CLIC3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human CLIC3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CLIC3 Antibody [NBP1-89465] - Simple Western lane view shows a specific band for CLIC3 in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CLIC3 Antibody [NBP1-89465] - Electropherogram image of the corresponding Simple Western lane view. CLIC3 antibody was used at 1:20 dilution on MCF-7 lysate(s) respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLIC3 Antibody [NBP1-89465] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: MCF-7
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CLIC3 Antibody [NBP1-89465] - Analysis in human cell lines MCF-7 and A-549 using anti-CLIC3 antibody. Corresponding CLIC3 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC3 Antibody [NBP1-89465] - Staining of human cerebral cortex shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC3 Antibody [NBP1-89465] - Staining of human esophagus shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CLIC3 Antibody [NBP1-89465] - Staining in human esophagus and cerebral cortex tissues using anti-CLIC3 antibody. Corresponding CLIC3 RNA-seq data are presented for the same tissues.