Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- A02965-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for PLAT detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C; for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Submitted references A multifunctional enolase mediates cytoadhesion and interaction with host plasminogen and fibronectin in Mycoplasma hyorhinis.
Erysipelothrix rhusiopathiae recruits host plasminogen via the major protective antigen SpaA.
Wang J, Yu Y, Li Y, Li S, Wang L, Wei Y, Wu Y, Pillay B, Olaniran AO, Chiliza TE, Shao G, Feng Z, Xiong Q
Veterinary research 2022 Mar 25;53(1):26
Veterinary research 2022 Mar 25;53(1):26
Erysipelothrix rhusiopathiae recruits host plasminogen via the major protective antigen SpaA.
Zhu W, Wang Y, Cai C, Li J, Wu C, Kang C, Jin M
FEMS microbiology letters 2017 Mar 1;364(5)
FEMS microbiology letters 2017 Mar 1;364(5)
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of PLAT using anti-PLAT antibody (A02965-1).PLAT was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PLAT Antibody (A02965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image