Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-31484 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDLIM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, HCT116. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inactivation of the putative ubiquitin-E3 ligase PDLIM2 in classical Hodgkin and anaplastic large cell lymphoma.
Wurster KD, Hummel F, Richter J, Giefing M, Hartmann S, Hansmann ML, Kreher S, Köchert K, Krappmann D, Klapper W, Hummel M, Wenzel SS, Lenz G, Janz M, Dörken B, Siebert R, Mathas S
Leukemia 2017 Mar;31(3):602-613
Leukemia 2017 Mar;31(3):602-613
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PDLIM2 Polyclonal Antibody (Product # PA5-31484). Sample (30 µg of whole cell lysate). Lane A: Hela. 10% SDS PAGE. PDLIM2 Polyclonal Antibody (Product # PA5-31484) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Loss of PDLIM2 expression in ALCL. ( a ) PDLIM2 mRNA expression analyses by quantitative (upper panel) and semi-quantitative (lower panel) PCR analyses in ALK-positive (K299, SU-DHL-1, DEL, JB6) and ALK-negative (FE-PD, Mac-1, Mac-2A, DL40) ALCL as well as T-cell leukemia-derived (Jurkat, KE-37, Molt-14, H9) control cell lines. ( a , upper panel) PDLIM2 mRNA expression was assessed by real-time quantitative PCR and calculated using the 2 -DeltaDeltaCt method. Expression in Jurkat cells was set as 1. Error bars denote 95% confidence intervals. One out of three independent experiments in shown. *** P