Submission file format

General information for file submission

• The file must be a delimited text file, comma separated or tab separated. E.g. In Excel: Save as "Text (Tab delimited) (*.txt)"
• The file must have a header row. Once you have uploaded the file you will be prompted to map your headers to the features in Antibodypedia.

Specific information for Antibody data

• The file must contain one column that provides a reference to a human antigen against which the antibody is reactive (i.e. Ensembl gene ID, Uniprot accession number, NCBI / EntrezGene ID or RefSeq peptide ID).
  • Please note that antibodies against non-human antigens may be listed in Antibodypedia if they possess reactivity against a cognate human protein. Human or Mouse gene accession numbers must be used for these antigens.
• Please note that applications must be assigned to appropriate columns ("Validated applications" / "Recommended applications without primary data") based on the presence or absence of user-accessible validation data (click here for additional information).
  • If antibodies are validated in more than one application they should be separated by commas (e.g., WB, IHC, FC and not WB IHC FC).

Antibody data example files

• Template file with headers only
• Example file with headers and example data

Available features

• Antibody type [Multiple choice, single] mandatory
  • Specify type of antibody.
  • Available options
    • Monoclonal
      • mAb
      • Monoclonal antibody
      • *monoclonal*
    • Polyclonal
      • pAb
      • Polyclonal antibody
      • *polyclonal*
    • Polyclonal (Antigen purified)
      • pAb (antigen-purified)
      • Antigen purified
      • *monospecific*
    • Recombinant
      • recAb
      • Recombinant antibody
      • *recombinant*
    • Other
      • Other
• Product name [Text] mandatory
  • The name of the binder. (Usually anti_[gene])
• Product number [Text] mandatory
  • Unique identifier of the binder.
• Provider product page [Link / URL] mandatory
  • Give the web link (URL) to the product page.
• Antibody clone number [Text]
  • Clone number for a monoclonal antibody produced by a clonal hybridoma culture.
• Antigen [Multiple choice, single]
  • Specify type of antigen used to generate antibody.
  • Available options
    • Purifed from natural sources
    • Recombinant full-length protein
    • Recombinant protein fragment
    • Synthetic peptide
    • Other
• Antigen [Text]
  • Specify type of antigen used to generate antibody.
• Antigen sequence [Sequence]
  • Specify the amino acid sequence for the antigen.
• Concentration [Number]
  • Specify the binder concentration in mg/ml (default), otherwise specify the unit.
• Conjugate [Multiple choice, many]
  • Enzyme or dye to which the binder is conjugated in this validation experiment.
  • Subcategories (e.g. AP, ALP) will be recognized in spreadsheet cells and entered into Antibodypedia as supercategories (e.g. Alkaline phosphatase).
  • Starred subcategories (e.g. *350, *405) can accommodate wildcard values (in other words, free text substitutions for the asterisk such that ‘*405’ may be replaced by ‘Alexa 405’). Please note that starred subcategories will also appear in Antibodypedia as the corresponding supercategory (e.g. Blue dyes).
  • Lists of multiple options should have choices separated by , (comma).
  • Available options
    • Unconjugated
    • Agarose/Sepharose
      • Agarose Immobilized
      • Agarose*
      • Sepharose*
    • Alkaline Phosphatase
      • AP
      • ALP
    • Biotin
      • Biotin*
    • Horseradish Peroxidase
      • HRP
    • Blue dye
      • Pacific Blue
      • *350
      • *405
    • Green dye
      • FITC
      • Cyanine 2
      • Cy2*
      • *488
    • Yellow dye
      • R-Phycoerythrin
      • PE
      • Cyanine 3
      • Cy3*
      • *532
      • *547
      • *549
      • *550
    • Orange dye
      • TRITC
      • *568
    • Red dye
      • Texas Red
      • Allophycocyanin
      • PerCP
      • Cyanine 5
      • Cy5*
      • *594
      • *633
      • *647
      • *649
      • *650
    • Far-Red dye
      • Cyanine 7
      • Cy7*
    • Near infrared dye
      • *680
      • *700
      • *755
    • Infrared dye
      • IR800CW
      • *800
    • Other fluorescent conjugates
    • Other non-fluorescent conjugates
• Description [Long text]
  • Short description of the binder. e.g., antibody affinity purified from serum; antibody purified from hybridoma supernatant.
• Epitope [Text]
  • Specify the amino acid sequence for the epitope.
• Handling [Text]
  • Indicate how the binder should be handled.
• Host [Multiple choice, single]
  • Host in which the antibody was generated.
  • Available options
    • Mouse
    • Rat
    • Goat
    • Rabbit
    • Bovine
      • Cow
    • Canine
      • Dog
    • Chicken/Avian
      • Chicken
      • Avian
    • Donkey
    • Feline
      • Cat
    • Guinea Pig
    • Hamster
    • Horse
    • Human
    • Porcine
      • Pig
    • Sheep
    • Simian
      • Primate
      • Monkey
    • Other
• Isotype [Multiple choice, single]
  • Specify the isotype of the binder. Choose from IgA, IgD, IgE, IgG, and IgM.
  • Available options
    • IgA
      • IgA*
    • IgD
      • IgD*
    • IgE
      • IgE*
    • IgG
      • IgG*
    • IgM
      • IgM*
    • IgY
      • IgY*
• Reactivity [Multiple choice, many]
  • Species with which the binder reacts.
  • Lists of multiple options should have choices separated by , (comma).
  • Available options
    • Human
      • Human*
    • Mouse
      • Mouse*
    • Rat
      • Rat*
    • Bacteria
      • Bacteria*
    • Bovine
      • Bovine*
      • Cow*
    • Canine
      • Canine*
      • Dog*
    • Chicken/Avian
      • Chicken*
      • Avian*
    • Donkey
      • Donkey*
    • Drosophila
      • Drosophila*
    • Feline
      • Feline*
      • Cat*
    • Goat
      • Goat*
    • Guinea Pig
      • Guinea Pig*
    • Hamster
      • Hamster*
    • Horse
      • Horse*
    • Porcine
      • Porcine*
      • Pig*
    • Rabbit
      • Rabbit*
    • Sheep
      • Sheep*
    • Simian
      • Simian*
      • Primate*
      • Monkey*
    • Viral
      • Viral*
    • Xenopus
      • Xenopus*
    • Yeast
      • Yeast*
    • Zebrafish
      • Zebrafish*
      • Zebra fish*
      • Danio rerio*
      • Zfsh*
    • Other
      • Other*
• Recommended applications without primary data [Multiple choice, many]
  • Specify in which applications the antibody is functional.
  • Subcategories (e.g. Westernblot, WB, Western blotting, Western blotting) will be recognized in spreadsheet cells and entered into Antibodypedia as supercategories (e.g. Western blot).
  • Starred subcategories (e.g. IH-*, IHC-*, Immunohistochemistry-*) can accommodate wildcard values (in other words, free text substitutions for the asterisk such that 'IHC-*' may be replaced by 'IHC-paraffin'). Please note that starred subcategories will also appear in Antibodypedia as the corresponding supercategory (e.g. Immunohistochemistry).
  • Lists of multiple options should have choices separated by , (comma).
  • Available options
    • Western blot
      • *Western blot*
      • *Westernblot*
      • WB*
      • Immunoblot
    • ELISA
      • *ELISA*
      • Peptide ELISA
      • EIA
    • Immunofluorescence
      • *Immunofluorescence*
      • IF*
    • Immunoprecipitation
      • Immunoprecipitation*
      • IP*
    • Immunohistochemistry
      • *Immunohistochemistry*
      • IH*
      • IHC*
    • Flow cytometry
      • *Flow cytometry*
      • Flow Cyt*
      • Flow-Cyt*
      • FACS*
      • FC*
    • Immunocytochemistry
      • *Immunocytochemistry*
      • ICC*
    • Protein assay
      • *Protein assay*
      • *Protein array*
      • PA*
    • Blocking/Neutralizing
      • *Blocking/Neutraliz*
      • *Neutralization*
      • Blocking*
      • *Inhibition*
      • Neu*
      • Block*
    • Immunoelectron microscopy
      • *Electron microscopy*
      • *Immunoelectron microscopy*
      • EM*
      • IEM*
    • Functional assay
      • *Functional*
      • FA
      • FN*
      • CA
      • FuncS
    • Gel shift
      • *Gel shift*
      • *shift assay*
      • GS*
      • EMSA*
    • Radioimmunoassay
      • *Radioimmunoassay*
      • RIA*
      • IRIA*
    • Proximity ligation assay
      • PLA*
    • Chromatin immunoprecipitation
      • Chromatin immunoprecipitation*
      • ChIP*
    • Reverse phase protein array
      • RPPA*
    • Other assay
      • Other*
• Storage [Text]
  • Indicate how the binder should be stored.
• Validated applications [Multiple choice, many]
  • Specify in which applications the antibody is functional.
  • Subcategories (e.g. Westernblot, WB, Western blotting, Western blotting) will be recognized in spreadsheet cells and entered into Antibodypedia as supercategories (e.g. Western blot).
  • Starred subcategories (e.g. IH-*, IHC-*, Immunohistochemistry-*) can accommodate wildcard values (in other words, free text substitutions for the asterisk such that 'IHC-*' may be replaced by 'IHC-paraffin'). Please note that starred subcategories will also appear in Antibodypedia as the corresponding supercategory (e.g. Immunohistochemistry).
  • Lists of multiple options should have choices separated by , (comma).
  • Available options
    • Western blot
      • *Western blot*
      • *Westernblot*
      • WB*
      • Immunoblot
    • ELISA
      • *ELISA*
      • Peptide ELISA
      • EIA
    • Immunofluorescence
      • *Immunofluorescence*
      • IF*
    • Immunoprecipitation
      • Immunoprecipitation*
      • IP*
    • Immunohistochemistry
      • *Immunohistochemistry*
      • IH*
      • IHC*
    • Flow cytometry
      • *Flow cytometry*
      • Flow Cyt*
      • Flow-Cyt*
      • FACS*
      • FC*
    • Immunocytochemistry
      • *Immunocytochemistry*
      • ICC*
    • Protein assay
      • *Protein assay*
      • *Protein array*
      • PA*
    • Blocking/Neutralizing
      • *Blocking/Neutraliz*
      • *Neutralization*
      • Blocking*
      • *Inhibition*
      • Neu*
      • Block*
    • Immunoelectron microscopy
      • *Electron microscopy*
      • *Immunoelectron microscopy*
      • EM*
      • IEM*
    • Functional assay
      • *Functional*
      • FA
      • FN*
      • CA
      • FuncS
    • Gel shift
      • *Gel shift*
      • *shift assay*
      • GS*
      • EMSA*
    • Radioimmunoassay
      • *Radioimmunoassay*
      • RIA*
      • IRIA*
    • Proximity ligation assay
      • PLA*
    • Chromatin immunoprecipitation
      • Chromatin immunoprecipitation*
      • ChIP*
    • Reverse phase protein array
      • RPPA*
    • Other assay
      • Other*
• Vial size [Integer]
  • Specify the vial size in µl (default), otherwise specify the unit.

General information for file submission

• The file must be a delimited text file, comma separated or tab separated. E.g. In Excel: Save as "Text (Tab delimited) (*.txt)"
• The file must have a header row. Once you have uploaded the file you will be prompted to map your headers to the features in Antibodypedia.

Specific information for Validation data

• The file must contain one column mapping antibody product numbers to the validation data contained in the other columns (see template files below).
• Separate files for validation data upload should be created for each application type.

Validation data example files

Validation type: Blocking/NeutralizingChromatin ImmunoprecipitationELISAFlow cytometryFunctional assayGel shiftImmunocytochemistryImmunoelectron microscopyImmunohistochemistryImmunoprecipitationOther assayProtein arrayProximity ligation assayRadioimmunoassayReverse phase protein arrayWestern blot
Validation method: Genetic validationOrthogonal validationIndependent antibody validationRecombinant expression validationCapture MS validationOther validation

• Template file with headers only
• Example file with headers and example data

Available features

• Main image [Image / URL to image] mandatory
  • Main image which shows the outcome of the validation experiment. Image URL if submitted in batch format. (Most formats supported e.g. JPG, GIF, TIF, PNG, BMP)
• Experimental details [Long text] mandatory
  • Text which describes the details of the validation experiment and explains the attached image.
• Validation category [Multiple choice, single] mandatory
  • Classify the application outcome according to literature and other known facts.
  • Available options
    • Supportive
    • Non-supportive
• Validated as part of the Validation Initative [Multiple choice, many]
  • Validation is submitted as part of the Validation Initiative.
  • Lists of multiple options should have choices separated by , (comma).
  • Available options
    • Yes
• Additional image [Image / URL to image]
  • Additional image which shows the outcome of the validation experiment in complement to the main image. Image URL if submitted in batch format. (Most formats supported e.g. JPG, GIF, TIF, PNG, BMP)
• Sample type [Text]
  • Please enter type of sample processed, including organism. If cells, please specify cell line or, if primary cells, their tissue of origin. If tissue, please specify tissue type.
• KD Reagent Type [Multiple choice, single]
  • Select type of knockdown reagent, i.e siRNA or CRISPR.
  • Available options
    • CRISPR
    • siRNA
• Downregulation [Multiple choice, single]
  • Downregulation in knockdown validation. Determines validation category.
  • Available options
    • >75%
    • >50%
    • <50%
• KD Reagent Prod no [Text]
  • Enter the siRNA/CRISPR product number.
• KD Reagent Prod page [Link / URL]
  • Enter URL for Knockdown Reagent product web page.
• KD Reagent Provider [Text]
  • Enter the siRNA/CRISPR provider name.
• KD Reagent Page 2 [Link / URL]
  • Enter URL for second Knockdown Reagent product web page.
• KD Reagent Prod no 2 [Text]
  • Enter the siRNA/CRISPR product number of the second reagent used.
• KD Reagent Page 3 [Link / URL]
  • Enter URL for third Knockdown Reagent product web page.
• KD Reagent Prod no 3 [Text]
  • Enter the siRNA/CRISPR product number of the third reagent used.
• Validation comment [Text]
  • Details about the selected validation category.
• Antibody Lot Number [Text]
  • The lot number on the affinity reagent used.
• Primary Ab dilution [Text]
  • Dilution used.
• Other comments [Long text]
  • Further comments and information about the validation.
• Conjugate [Multiple choice, single]
  • Enzyme or dye to which the binder is conjugated in this validation experiment.
  • Subcategories (e.g. AP, ALP) will be recognized in spreadsheet cells and entered into Antibodypedia as supercategories (e.g. Alkaline phosphatase).
  • Starred subcategories (e.g. *350, *405) can accommodate wildcard values (in other words, free text substitutions for the asterisk such that ‘*405’ may be replaced by ‘Alexa 405’). Please note that starred subcategories will also appear in Antibodypedia as the corresponding supercategory (e.g. Blue dyes).
  • Available options
    • Unconjugated
    • Agarose/Sepharose
      • Agarose Immobilized
      • Agarose*
      • Sepharose*
    • Alkaline Phosphatase
      • AP
      • ALP
    • Biotin
      • Biotin*
    • Horseradish Peroxidase
      • HRP
    • Blue dye
      • Pacific Blue
      • *350
      • *405
    • Green dye
      • FITC
      • Cyanine 2
      • Cy2*
      • *488
    • Yellow dye
      • R-Phycoerythrin
      • PE
      • Cyanine 3
      • Cy3*
      • *532
      • *547
      • *549
    • Orange dye
      • TRITC
      • *568
    • Red dye
      • Texas Red
      • Allophycocyanin
      • PerCP
      • Cyanine 5
      • Cy5*
      • *647
      • *649
    • Far-Red dye
      • Cyanine 7
      • Cy7*
    • Near infrared dye
      • *680
      • *700
    • Infrared dye
      • IR800CW
      • *800
    • Other fluorescent conjugates
    • Other non-fluorescent conjugates
• Secondary Ab [Link / URL]
  • Link to secondary Ab used in validation.
• Secondary Ab dilution [Text]
  • 2nd dilution used.
• Protocol [Link / URL]
  • URL to used protocol.
• Appendix [File / URL to file]
  • PDF or other appendix file.

General information for file submission

• The file must be a delimited text file, comma separated or tab separated. E.g. In Excel: Save as "Text (Tab delimited) (*.txt)"
• The file must have a header row. Once you have uploaded the file you will be prompted to map your headers to the features in Antibodypedia.

Specific information for References

• The file must contain one column mapping references to their corresponding antibodies (i.e., antibody product number).

References example files

• Template file with headers only
• Example file with headers and example data

Available features

• Reference [Text] mandatory
  • Pubmed ID or Digital Object Identifier (DOI).
• Referenced application [Multiple choice, many]
  • The application where the antibody has been used according to the reference.
  State the applicaiton where the antibody has been successfully used
  • Subcategories (e.g. Westernblot, WB, Western blotting, Western blotting) will be recognized in spreadsheet cells and entered into Antibodypedia as supercategories (e.g. Western blot).
  • Starred subcategories (e.g. IH-*, IHC-*, Immunohistochemistry-*) can accommodate wildcard values (in other words, free text substitutions for the asterisk such that 'IHC-*' may be replaced by 'IHC-paraffin'). Please note that starred subcategories will also appear in Antibodypedia as the corresponding supercategory (e.g. Immunohistochemistry).
  • Lists of multiple options should have choices separated by , (comma).
  • Available options
    • Western blot
      • *Western blot*
      • *Westernblot*
      • WB*
      • Immunoblot
    • ELISA
      • *ELISA*
      • Peptide ELISA
      • EIA
    • Immunofluorescence
      • *Immunofluorescence*
      • IF*
    • Immunoprecipitation
      • Immunoprecipitation*
      • IP*
    • Immunohistochemistry
      • *Immunohistochemistry*
      • IH*
      • IHC*
    • Flow cytometry
      • *Flow cytometry*
      • Flow Cyt*
      • Flow-Cyt*
      • FACS*
      • FC*
    • Immunocytochemistry
      • *Immunocytochemistry*
      • ICC*
    • Protein assay
      • *Protein assay*
      • *Protein array*
      • PA*
    • Blocking/Neutralizing
      • *Blocking/Neutraliz*
      • *Neutralization*
      • Blocking*
      • *Inhibition*
      • Neu*
      • Block*
    • Immunoelectron microscopy
      • *Electron microscopy*
      • *Immunoelectron microscopy*
      • EM*
      • IEM*
    • Functional assay
      • *Functional*
      • FA
      • FN*
      • CA
      • FuncS
    • Gel shift
      • *Gel shift*
      • *shift assay*
      • GS*
      • EMSA*
    • Radioimmunoassay
      • *Radioimmunoassay*
      • RIA*
      • IRIA*
    • Proximity ligation assay
      • PLA*
    • Chromatin immunoprecipitation
      • Chromatin immunoprecipitation*
      • ChIP*
    • Reverse phase protein array
      • RPPA*
    • Other assay
      • Other*
• Sample [Multiple choice, many]
  • Origin of sample used in application.
  • Lists of multiple options should have choices separated by , (comma).
  • Available options
    • Human
      • Human*
    • Mouse
      • Mouse*
    • Rat
      • Rat*
    • Bacteria
      • Bacteria*
    • Bovine
      • Bovine*
      • Cow*
    • Canine
      • Canine*
      • Dog*
    • Chicken/Avian
      • Chicken*
      • Avian*
    • Donkey
      • Donkey*
    • Drosophila
      • Drosophila*
    • Feline
      • Feline*
      • Cat*
    • Goat
      • Goat*
    • Guinea Pig
      • Guinea Pig*
    • Hamster
      • Hamster*
    • Horse
      • Horse*
    • Porcine
      • Porcine*
      • Pig*
    • Rabbit
      • Rabbit*
    • Sheep
      • Sheep*
    • Simian
      • Simian*
      • Primate*
      • Monkey*
    • Viral
      • Viral*
    • Xenopus
      • Xenopus*
    • Yeast
      • Yeast*
    • Zebrafish
      • Zebrafish*
      • Zebra fish*
      • Danio rerio*
      • Zfsh*
    • Other
      • Other*