Validation Initiative

The Antibodypedia Validation Initiative

An International Working Group on Antibody Validation (IWGAV) has made an effort to standardize the best practice for validating antibodies. This has resulted in a publication presenting guidelines called the pillars of antibody validation (Ref: Uhlen, M., et al. (2016). A proposal for validation of antibodies. Nature Methods.). The pillars presented are directed to both users and producers of antibodies.

The five pillars are:
  1. Genetic strategies
  2. Orthogonal strategies
  3. Independent antibody strategies
  4. Tagged proteins
  5. Immunocapture-Mass spectrometry

A public-private partnership

The research community and antibody providers collaborate to improve quality of affinity reagents.

Are you a scientist whom uses antibodies in your daily research?

Please upload your results to contribute to the quality assessment of affinity reagents. We are working on getting antibody suppliers on board to be able to reward you for your efforts. If the antibody is within the initiative, you will get rewarded for the first positive validation submitted for that antibody.*

Antibodies that are participating in the initiative are marked with a faded symbol on Antibodypedia. If the symbol is filled, at least one validation has been uploaded and published on Antibodypedia.

Qualification

To qualify for a reward, the experimental setup must be at recommended conditions.

Submission

The submission only takes a few minutes, and you provide great help to all fellow antibody users.

Take the opportunity to share your data and get rewarded for it!**

Rewards issued by supplier

  • €400 or $400 for whichever applies for antibody in validation
  • Please note that the levels may be subject to change and that company specific details may apply. The rewards are valid for purchase from the same product family as they are issued for, e.g. an antibody reward can be used to buy a new antibody but no other products.

    Click here to submit an antibody validation: validation submission

    You may be prompted for verification of purchase for the reagents that you ask to be rewarded.

    *Please note that only the first validation for each binder and validation pillar will be eligible for a reward. The submitted validation data will be reviewed by trained personnel and will be awarded at our sole discretion (Antibodypedia and company). The reward will be sent to the submitter through Antibodypedia, to be used at participating partners' site.

    **If you get rewarded for the uploaded data, the supplier(s) is granted free access to the image and the experimental details for the validation.

    Participating partners antibodies

  • Atlas Antibodies
  • GeneTex
  • Novus Biologicals
  • R&D Systems
    1. Genetic strategies
    2. Orthogonal strategies
    3. Independent antibody strategies
    4. Tagged proteins
    5. Immunocapture-Mass spectrometry