600-401-948

antibody from Rockland Immunochemicals, Inc.

Targeting: CASP5 ICE(rel)III

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-948

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-948, RRID:AB_1961494

Product name

Anti-Cas5 (RABBIT) Antibody - 600-401-948

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-hCas5 antibody shows detection of a band ~125 kDa in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kDa). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA. Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.

Validation comment

Western Blot