Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-406-103 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-406-103, RRID:AB_217579
- Product name
- Anti-Collagen Type I (RABBIT) Antibody Biotin Conjugated - 600-406-103
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Inhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis.
Fibrocytes: A Critical Review and Practical Guide.
Blockade of PD-1/PD-L1 Pathway Enhances the Antigen-Presenting Capacity of Fibrocytes.
Effect of Orento, a Traditional Japanese Medicine, on IL-6, IL-8 Secretion, Type 1 Collagen Production and Alkaline Phosphatase Secretion in the Human Osteosarcoma Cell Line Saos-2.
A high-content, in vitro cardiac fibrosis assay for high-throughput, phenotypic identification of compounds with anti-fibrotic activity.
S100A4 Protein Is Essential for the Development of Mature Microfold Cells in Peyer's Patches.
Monocyte-derived fibrocytes elimination had little contribution on liver fibrosis.
Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis.
Cortactin promotes exosome secretion by controlling branched actin dynamics.
MicroRNA-29c regulates apoptosis sensitivity via modulation of the cell-surface death receptor, Fas, in lung fibroblasts.
Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis.
ZBTB17 (MIZ1) Is Important for the Cardiac Stress Response and a Novel Candidate Gene for Cardiomyopathy and Heart Failure.
Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer.
CXC-type chemokines promote myofibroblast phenoconversion and prostatic fibrosis.
Bone marrow-derived progenitor cells in pulmonary fibrosis.
D'Alessandro-Gabazza CN, Yasuma T, Kobayashi T, Toda M, Abdel-Hamid AM, Fujimoto H, Hataji O, Nakahara H, Takeshita A, Nishihama K, Okano T, Saiki H, Okano Y, Tomaru A, Fridman D'Alessandro V, Shiraishi M, Mizoguchi A, Ono R, Ohtsuka J, Fukumura M, Nosaka T, Mi X, Shukla D, Kataoka K, Kondoh Y, Hirose M, Arai T, Inoue Y, Yano Y, Mackie RI, Cann I, Gabazza EC
Nature communications 2022 Mar 23;13(1):1558
Nature communications 2022 Mar 23;13(1):1558
Fibrocytes: A Critical Review and Practical Guide.
Reinhardt JW, Breuer CK
Frontiers in immunology 2021;12:784401
Frontiers in immunology 2021;12:784401
Blockade of PD-1/PD-L1 Pathway Enhances the Antigen-Presenting Capacity of Fibrocytes.
Afroj T, Mitsuhashi A, Ogino H, Saijo A, Otsuka K, Yoneda H, Tobiume M, Nguyen NT, Goto H, Koyama K, Sugimoto M, Kondoh O, Nokihara H, Nishioka Y
Journal of immunology (Baltimore, Md. : 1950) 2021 Mar 15;206(6):1204-1214
Journal of immunology (Baltimore, Md. : 1950) 2021 Mar 15;206(6):1204-1214
Effect of Orento, a Traditional Japanese Medicine, on IL-6, IL-8 Secretion, Type 1 Collagen Production and Alkaline Phosphatase Secretion in the Human Osteosarcoma Cell Line Saos-2.
Oh H, Masuno K, Okusa N, Makita Y, Fujiwara SI, Imamura Y
Medicines (Basel, Switzerland) 2020 Sep 25;7(10)
Medicines (Basel, Switzerland) 2020 Sep 25;7(10)
A high-content, in vitro cardiac fibrosis assay for high-throughput, phenotypic identification of compounds with anti-fibrotic activity.
Palano G, Jansson M, Backmark A, Martinsson S, Sabirsh A, Hultenby K, Åkerblad P, Granberg KL, Jennbacken K, Müllers E, Hansson EM
Journal of molecular and cellular cardiology 2020 May;142:105-117
Journal of molecular and cellular cardiology 2020 May;142:105-117
S100A4 Protein Is Essential for the Development of Mature Microfold Cells in Peyer's Patches.
Kunimura K, Sakata D, Tun X, Uruno T, Ushijima M, Katakai T, Shiraishi A, Aihara R, Kamikaseda Y, Matsubara K, Kanegane H, Sawa S, Eberl G, Ohga S, Yoshikai Y, Fukui Y
Cell reports 2019 Nov 26;29(9):2823-2834.e7
Cell reports 2019 Nov 26;29(9):2823-2834.e7
Monocyte-derived fibrocytes elimination had little contribution on liver fibrosis.
Ozono Y, Shide K, Toyoshima F, Takaishi Y, Tsuchimochi M, Kamiunten A, Kameda T, Nakamura K, Miike T, Kusumoto K, Iwakiri H, Hasuike S, Nagata K, Sawaguchi A, Shimoda K
Hepatobiliary & pancreatic diseases international : HBPD INT 2019 Aug;18(4):348-353
Hepatobiliary & pancreatic diseases international : HBPD INT 2019 Aug;18(4):348-353
Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis.
Heukels P, van Hulst JAC, van Nimwegen M, Boorsma CE, Melgert BN, van den Toorn LM, Boomars KAT, Wijsenbeek MS, Hoogsteden H, von der Thüsen JH, Hendriks RW, Kool M, van den Blink B
Respiratory research 2018 May 10;19(1):90
Respiratory research 2018 May 10;19(1):90
Cortactin promotes exosome secretion by controlling branched actin dynamics.
Sinha S, Hoshino D, Hong NH, Kirkbride KC, Grega-Larson NE, Seiki M, Tyska MJ, Weaver AM
The Journal of cell biology 2016 Jul 18;214(2):197-213
The Journal of cell biology 2016 Jul 18;214(2):197-213
MicroRNA-29c regulates apoptosis sensitivity via modulation of the cell-surface death receptor, Fas, in lung fibroblasts.
Matsushima S, Ishiyama J
American journal of physiology. Lung cellular and molecular physiology 2016 Dec 1;311(6):L1050-L1061
American journal of physiology. Lung cellular and molecular physiology 2016 Dec 1;311(6):L1050-L1061
Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis.
Collins SL, Chan-Li Y, Oh M, Vigeland CL, Limjunyawong N, Mitzner W, Powell JD, Horton MR
JCI insight 2016 Apr 7;1(4):e83116
JCI insight 2016 Apr 7;1(4):e83116
ZBTB17 (MIZ1) Is Important for the Cardiac Stress Response and a Novel Candidate Gene for Cardiomyopathy and Heart Failure.
Buyandelger B, Mansfield C, Kostin S, Choi O, Roberts AM, Ware JS, Mazzarotto F, Pesce F, Buchan R, Isaacson RL, Vouffo J, Gunkel S, Knöll G, McSweeney SJ, Wei H, Perrot A, Pfeiffer C, Toliat MR, Ilieva K, Krysztofinska E, López-Olañeta MM, Gómez-Salinero JM, Schmidt A, Ng KE, Teucher N, Chen J, Teichmann M, Eilers M, Haverkamp W, Regitz-Zagrosek V, Hasenfuss G, Braun T, Pennell DJ, Gould I, Barton PJ, Lara-Pezzi E, Schäfer S, Hübner N, Felkin LE, O'Regan DP, Brand T, Milting H, Nürnberg P, Schneider MD, Prasad S, Petretto E, Knöll R
Circulation. Cardiovascular genetics 2015 Oct;8(5):643-52
Circulation. Cardiovascular genetics 2015 Oct;8(5):643-52
Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer.
Zhang H, Maric I, DiPrima MJ, Khan J, Orentas RJ, Kaplan RN, Mackall CL
Blood 2013 Aug 15;122(7):1105-13
Blood 2013 Aug 15;122(7):1105-13
CXC-type chemokines promote myofibroblast phenoconversion and prostatic fibrosis.
Gharaee-Kermani M, Kasina S, Moore BB, Thomas D, Mehra R, Macoska JA
PloS one 2012;7(11):e49278
PloS one 2012;7(11):e49278
Bone marrow-derived progenitor cells in pulmonary fibrosis.
Hashimoto N, Jin H, Liu T, Chensue SW, Phan SH
The Journal of clinical investigation 2004 Jan;113(2):243-52
The Journal of clinical investigation 2004 Jan;113(2):243-52
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 µg per lane. Protein staining shown below each blot depicts equal protein loading. Primary antibody: anti-Collagen I antibody at 0.22 µg/10 ml for overnight at 4°C. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 µg/10 ml for overnight at 4°C. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band(s): none.
- Validation comment
- Western Blot