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Learn600-401-B05
antibody from Rockland Immunochemicals, Inc.
Targeting: NEDD4
KIAA0093, MGC176705, NEDD4-1, RPF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 600-401-B05 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-B05, RRID:AB_2266923
- Product name
- Anti-NEDD4 (RABBIT) Antibody - 600-401-B05
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations.
Kuhn TM, Little SC, Capelson M
Bio-protocol 2020 Jul 5;10(13):e3673
Bio-protocol 2020 Jul 5;10(13):e3673
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. The blot was blocked with B501-0500 5% BLOTTO overnight at 4°C. Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blotting. ECL was used for detection.
- Validation comment
- Western Blot
