600-401-483
antibody from Rockland Immunochemicals, Inc.
Targeting: FBXW11
BTRC2, BTRCP2, Fbw11, Fbw1b, FBXW1B, Hos, KIAA0696
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-483 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-483, RRID:AB_2102593
- Product name
- Anti-ßTrCP2 (RABBIT) Antibody - 600-401-483
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-bTrCP2 antibody shows detection of mouse and human bTrCP2 (arrowhead) in NIH3T3 (lane 1) and 293 (lane 2) whole cell lysates, respectively. The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted. The identity of faint higher molecular weight bands is not known. The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C. Detection occurred using HRP conjugated Goat-anti-Rabbit IgG (p/n 611-103-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 1 h at 4 °C.
- Validation comment
- Western Blot