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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 109-401-312S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#109-401-312S, RRID:AB_11181731
- Product name
- Anti-Human IL-10 (RABBIT) Antibody - 109-401-312S
- Antibody type
- Polyclonal
- Vial size
- 25 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Rockland Anti IL-10 whole antiserum (109-401-312) was tested against recombinant IL10. Recombinant IL-10 was run on a 4-20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA-TBS for 1 Hour 4oC and probed with p/n 109-401-312 diluted 1:200 in 3% BSA-TBS o/n 4oC. Primary antibody was detected with Gt anti Rb 611-103-122 Lot#21231 1:20,000 in MB-070 1 Hour 4oC using FemtoMax Super sensitive chemiluminescent substrate. Blot was imaged with VersaDoc Imaging system by Biorad. Other detection systems will yield similar results.
- Validation comment
- Western Blot
