Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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Comment
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- Product number
- 100-401-199 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-199, RRID:AB_2210716
- Product name
- Anti-THYMIDYLATE SYNTHASE (RABBIT) Antibody - 100-401-199
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Thymidylate synthase as a prognostic biomarker for locally advanced rectal cancer after multimodal treatment.
Conradi LC, Bleckmann A, Schirmer M, Sprenger T, Jo P, Homayounfar K, Wolff HA, Rothe H, Middel P, Becker H, Ghadimi MB, Beissbarth T, Liersch T
Annals of surgical oncology 2011 Sep;18(9):2442-52
Annals of surgical oncology 2011 Sep;18(9):2442-52
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:500, 1:1,000 and 1:2,000 fold dilutions of the antibody. Detection was made using HRP Goat-a-Rabbit IgG (611-1302) diluted 1:1,000 and color development using TMB (TMBM-100) substrate for approximately 4'.
- Validation comment
- Western Blot