600-401-H38S

antibody from Rockland Immunochemicals, Inc.

Targeting: STAT2 STAT113

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-H38S

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-H38S, RRID:AB_2614309

Product name

Anti-STAT2 pS734 (RABBIT) Antibody - 600-401-H38S

Antibody type

Polyclonal

Vial size

25 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Rabbit anti-STAT2pS734 antibody. Lane 1: U6A cells (STAT2 deficient) untreated. Lane 2: U6A cells reconstituted with STAT2 untreated. Lane 3: U6A cells, STAT2 treated with IFN? (1h). Lane 4: U6A cells, STAT2 + IFN? (4h). Lane 5: U6A cells, STAT2 + IFN? (8h). Lane 6: U6A cells reconstituted with S734A-STAT2 mutant untreated. Lane 7: U6A cells, S734A-STAT2 treated with IFN? (1h). Lane 8: U6A cells, S734A-STAT2 + IFN? (4h). Lane 9: U6A cells, S734A-STAT2 + IFN? (8h). Load: 20 µg per lane. Primary antibody: STAT2 pS734 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 97.9 kDa, ~110 kDa for STAT2pS734. Other band(s): Non specific 100kDa, STAT2, GAPDH loading control.

Validation comment

Western Blot