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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 100-4165N - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-4165N, RRID:AB_218046
- Product name
- Anti-NFKB p65 (Rel A) N-TERMINAL SPECIFIC (RABBIT) Antibody - 100-4165N
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-NFKB p65 N term antibody. Lane 1-4: PC3 cell lysate. Load: 20, 30, 40, and 50 ug per lane. Primary antibody: NFKB p65 antibody at 1:5000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~65kDa for NFKB p65. Other band(s): ~110kDa and ~45kDa.
- Validation comment
- Western Blot
