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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 100-401-F38 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-F38, RRID:AB_2610755
- Product name
- Anti-GRP78 (RABBIT) Antibody - 100-401-F38
- Antibody type
- Polyclonal
- Vial size
- 200 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of rabbit anti-Grp78 antibody. Lane 1: GRP78 recombinant protein. Lane 2: Human tissues. Lane 3: Dog tissues. Lane 4: Mouse tissues. Load: 10 µg per lane. Primary antibody: Grp78 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 72.4 kDa, ~75 kDa for Grp78. Other band(s): none.
- Validation comment
- Western Blot
