Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 600-401-B29 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-B29, RRID:AB_2107797
- Product name
- Anti-SMAD1 (RABBIT) Antibody - 600-401-B29
- Antibody type
- Polyclonal
- Vial size
- 100 µl
No comments: Submit comment
Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (p/n B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n 600-401-886) staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG (p/n 611-103-122) for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX
- Validation comment
- Western Blot