STJ22448

antibody from St John's Laboratory

Targeting: ZAP70 SRK, STD, ZAP-70

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

STJ22448

Provider

St John's Laboratory

Product name

Anti-Phospho-ZAP70-Y319 antibody (STJ22448)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody anti-Phospho-ZAP70-Y319 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NA


Epitope

NA

Isotype

IgG

Antibody clone number

NA

Vial size

NA

Concentration

NA

Storage

Store in a freezer at-20°C and avoid freeze-thaw cycles.

Handling

NA

Immunohistochemistry

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-ZAP70-Y319 antibody (STJ22448).

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

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Chromatin Immunoprecipitation

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ZAP70-Y319 polyclonal antibody (STJ22448). Western blot was performed from the immunoprecipitate using Phospho-ZAP70-Y319 polyclonal antibody (STJ22448) at a dilition of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes after serum-starvation overnight.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

Show more