100-401-407

antibody from Rockland Immunochemicals, Inc.

Targeting: NOTCH1 TAN1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-407

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-407, RRID:AB_2153490

Product name

Anti-NOTCH 1 (Cleaved N terminal) (Human specific) (RABBIT) Antibody - 100-401-407

Antibody type

Polyclonal

Vial size

200 µl

References

Submitted references

Podocyte-specific deletion of miR-146a increases podocyte injury and diabetic kidney disease.

Li X, Venkatesh I, Villanueva V, Wei H, Geraghty T, Rajagopalan A, Helmuth RW, Altintas MM, Faridi HM, Gupta V
Frontiers in medicine 2022;9:897188

---

Potassium depletion induces cellular conversion in the outer medullary collecting duct altering Notch signaling pathway.

Iervolino A, Prosperi F, De La Motte LR, Petrillo F, Spagnuolo M, D'Acierno M, Siccardi S, Perna AF, Christensen BM, Frische S, Capasso G, Trepiccione F
Scientific reports 2020 Mar 31;10(1):5708

---

Absence of miR-146a in Podocytes Increases Risk of Diabetic Glomerulopathy via Up-regulation of ErbB4 and Notch-1.

Lee HW, Khan SQ, Khaliqdina S, Altintas MM, Grahammer F, Zhao JL, Koh KH, Tardi NJ, Faridi MH, Geraghty T, Cimbaluk DJ, Susztak K, Moita LF, Baltimore D, Tharaux PL, Huber TB, Kretzler M, Bitzer M, Reiser J, Gupta V
The Journal of biological chemistry 2017 Jan 13;292(2):732-747

---

Hippo signaling is required for Notch-dependent smooth muscle differentiation of neural crest.

Manderfield LJ, Aghajanian H, Engleka KA, Lim LY, Liu F, Jain R, Li L, Olson EN, Epstein JA
Development (Cambridge, England) 2015 Sep 1;142(17):2962-71

---

Murine neural stem cells model Hunter disease in vitro: glial cell-mediated neurodegeneration as a possible mechanism involved.

Fusar Poli E, Zalfa C, D'Avanzo F, Tomanin R, Carlessi L, Bossi M, Nodari LR, Binda E, Marmiroli P, Scarpa M, Delia D, Vescovi AL, De Filippis L
Cell death & disease 2013 Nov 7;4(11):e906

---

Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling.

Harrison H, Simões BM, Rogerson L, Howell SJ, Landberg G, Clarke RB
Breast cancer research : BCR 2013 Mar 8;15(2):R21

---

Contrasting hypoxic effects on breast cancer stem cell hierarchy is dependent on ER-α status.

Harrison H, Rogerson L, Gregson HJ, Brennan KR, Clarke RB, Landberg G
Cancer research 2013 Feb 15;73(4):1420-33

---

Effect of Notch activation on the regenerative response to acute renal failure.

Gupta S, Li S, Abedin MJ, Wang L, Schneider E, Najafian B, Rosenberg M
American journal of physiology. Renal physiology 2010 Jan;298(1):F209-15

---

Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants.

Solomon A, Mian Y, Ortega-Cava C, Liu VW, Gurumurthy CB, Naramura M, Band V, Band H
Developmental biology 2008 Apr 15;316(2):191-9

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Rabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot. Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods. A reaction with diluted primary antibody was followed by washing; reaction with a 1:10,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122), and color development. Lane M: Mol wt markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc. Personal communication, Dr. Stacey Huppert.

Validation comment

Western Blot