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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 200-103-207S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-103-207S, RRID:AB_2610919
- Product name
- Anti-Alpha-2-MACROGLOBULIN (Human Plasma) (GOAT) Antibody Peroxidase Conjugated - 200-103-207S
- Antibody type
- Polyclonal
- Vial size
- 25 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Rockland Goat anti Alpha-2-Macroglobulin antibody (200-101-207 lot 20172) was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
- Validation comment
- Western Blot
